Validation of a one-step PCR assay for the molecular identification of Echinococcus granulosus sensu stricto G1–G3 genotype
نویسندگان
چکیده
منابع مشابه
First molecular characterization of Echinococcus granulosus (sensu stricto) genotype 1 among cattle in Sudan
BACKGROUND Echinococcus granulosus sensu lato (s.l.) is the causative agent of cystic echinococcosis (CE), which is a cosmopolitan zoonotic parasitic disease infecting humans and a wide range of mammalian species including cattle. Currently, little information is available on the genetic diversity of Echinococcus species among livestock in Sudan. In the present study, fifty (n = 50) hydatid cys...
متن کاملDiscrimination between E. granulosus sensu stricto, E. multilocularis and E. shiquicus Using a Multiplex PCR Assay
BACKGROUND Infections of Echinococcus granulosus sensu stricto (s.s), E. multilocularis and E. shiquicus are commonly found co-endemic on the Qinghai-Tibet plateau, China, and an efficient tool is needed to facilitate the detection of infected hosts and for species identification. METHODOLOGY/PRINCIPAL FINDINGS A single-tube multiplex PCR assay was established to differentiate the Echinococcu...
متن کاملMolecular characterization of a new microvariant of the G3 genotype for Echinococcus granulosus in water buffalo in Iran
In this study, molecular characterization of Echinococcus granulosus sample obtained from water buffalo originating from southwest of Iran was performed using comparative sequence analysis of cox1 mitochondrial gene. DNA was extracted from protoscoleces removed from hydatid cyst from the liver of a 2-year-old male buffalo slaughtered in Khuzestan province. Molecular and phylog...
متن کاملCases of Echinococcus granulosus Sensu Stricto Isolated from Polish Patients: Imported or Indigenous?
The cases of nine Polish patients with diagnosed cystic echinococcosis (CE) were examined. A total of nine isolates obtained postoperatively were investigated using PCR and sequencing. The mitochondrial region of nad1 gene was amplified. This PCR and sequencing analysis revealed the presence of Echinococcus canadensis G7 in seven patients and E. granulosus G1 in two patients. These data demonst...
متن کاملEchinococcus granulosus Sensu Stricto in Dogs and Jackals from Caspian Sea Region, Northern Iran
BACKGROUND The aim of the present study was genotyping of Echinococcus granulosus isolates from dogs and jackals in Mazandaran Province, northern Iran, and using partial sequence of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1). METHODS E. granulosus isolates (n = 15) were collected from 42 stray dogs and 16 jackals found in south of the Caspian Sea in northern Iran. After morp...
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ژورنال
عنوان ژورنال: Molecular Biology Reports
سال: 2019
ISSN: 0301-4851,1573-4978
DOI: 10.1007/s11033-019-04625-w